The test conducted on wheat (Triticum aestivum) is frequently used as a prescreening test for phytotoxicity studies in higher plants due to its advantages: quick results, simplified operative procedure, good reproducibility and repeatability and reduced costs. The laboratory methodology for the Triticum test (Constantinescu method) is applied by different authors with some modifications imposed by the properties of the tested substance (structural formula, purity, solubility in water or other solvents, stability in water or solvent, and biodegradability). The potential of the genus Triticum to be used for toxicity tests is demonstrated by the numerous research studies that evaluated different categories of substances determining different morphological, physiological, biochemical and genetic parameters.