Pepper production is found from the humid tropics, to the dry deserts, to the cool temperate climates. The ability of pepper to thrive under this range of climatic conditions has rendered it a common crop worldwide. USA, China, India and Turkey are major producer in the world. The production of pepper has done in two different ways as industrial and fresh consumption. Industrial production are sauce, canned food and powder form or red pepper flakes after drying. In industrial pepper cultivation for spice production, harvesting the fruits is time consuming and high cost requiring process. The pedicel is tightly attached with the calyx to the fruit pod in most pepper cultivar. For this reason fruit detachment force is an important for pepper breeding programs. In present study, it is aimed that determination of fruit detachment force of some breeding lines for breeding program. Total 145 pepper lines were used and fruit detachment force of pepper lines were observed as newton. As results of study, fruit detachment of pepper lines varied between 0.56-4.41 N. Variation is useful for pepper breeding programs with regard to fruit detachment force.