期刊名称：Journal of Materials and Environmental Science
印刷版ISSN：2028-2508
出版年度：2017
卷号：8
期号：9
页码：3247-3251
出版社：University of Mohammed Premier Oujda
摘要：Dayet-Aaoua lake (DA) is a freshwater aquatic ecosystem of ecological interest, located at a middle Atlas mountains under humid hydrographic basin (Ifran, Morocco) (33° 39′ 10″ N, 5° 02′ 30″ W). This work deals on the toxicity assessment of Microcystis aeruginosa strain isolated from DA lake, using both Artemia bioassay and the high-performance liquid chromatography (HPLC) equipment provided with a photodiode array detector (PDA. HPLC-PDA identification and quantification of hepatotoxins cyanotoxins (Microcystins). The toxicity of the cultural Microcystis biomass evaluated by Artemia bioassay revealed a positive letal concentions (LC) (24-h LC50 = 9.01 mg mL-1 and 40-h LC50 = 6.87 mg mL-1). Microcystins (MC) variants selected by HPLC-PDA showed a high MC content concentration (185.56 μg g-1 Dry weight) which confirmed the toxicity assessed. Four MC variants were clearly identified in the DA biomass (MC-WR, MC-RR, DM-WR, MC- YR). For the first time in this natural lake, the obtained results confirms the presence of producing cyanotoxins Microcystis strains, detection of various microcystin congeners and quantification of high amount of MCs. In such ecological ecosystem, the presence of toxic Microcystis, especially in favorable periods of cyanobacteria bloom proliferations, may be regarded as an environmental threat and health hazards. Therefore, an establishment of cyanotoxins monitoring program in this natural lake is highly recommended.
