期刊名称：Journal of Materials and Environmental Science
印刷版ISSN：2028-2508
出版年度：2018
卷号：9
期号：9
页码：2529-2538
出版社：University of Mohammed Premier Oujda
摘要：The effect of different growth regulators and elicitors on the in vitro production of itsmain constituents; cucurbitacins E and I, in the callus of Ecballium elaterium growingin Egypt was investigated. The suspension culture was supplemented with biotic stresssubstance like chitosan, abiotic elicitors like acetic acid, citric acid and salicylic acidand drought stress agents like mannitol. The accumulation of cucurbitacins E and I incallus and media were analyzed using high performance liquid chromatography. Thehighest content of Cu E (3.6 mg/g DW) and Cu I (1.3 mg/g DW) were achieved whenthe media supplemented with a combination of 5.0 mg/L NAA 1.0 mg/L BA and 0.1mg/L NAA 1.0 mg/L BA, respectively. The use of chitosan (1g/L) was found to givethe highest concentration of cucurbitacin E (2.6 mg % w/w) in the media, while the useof 0.5 mM of salicylic acid yielded 1.3 mg/g dry weight of cucurbitacin E in the callus.On the other hand; the highest concentration of cucurbitacin I released to the media (2.4mg % w/w) and in the callus (0.5 mg/g DW) was obtained by using mannitol 20% aselicitor. Hereby we report the studying of the effects of different plant growth regulatorsand elicitors on the production of cucurbitacins in E. elaterium plant. Elicitation studieshave a promising results in increasing yields of the secondary metabolites along withdecreasing the production costs.
