Improving good employee performance can have an impact on company success, employees are always required to work optimally where good or poor employee performance can affect overall company income. One of the most important in improving employee performance is Intrinsic Motivation. This study aims to determine and analyze the influence of Islamic leadership, emotional quotient, intrinsic motivation on employee performance. The population of this research is all employees at Sultan Agung Islamic Hospital Semarang, Indonesia. The number of samples studied in this study was 100 respondents with a purposive sampling technique, that is by determining specific characteristics in accordance with the research objectives. Data analysis in this study used multiple regression analysis. The results of this study indicate that there is an influence between Islamic leadership on employee performance, emotional quotient on employee performance, intrinsic motivation on employee performance, Islamic leadership on intrinsic motivation, and emotional quotient on intrinsic motivation.