A unidirectional dual-band coplanar waveguide fed antenna (DB-CPWFA) loaded with a reflector is presented in this paper. The reflector is made of an electric ground plane, a dielectric substrate, and artificial magnetic conductor (AMC) which shows an effective dual operational bandwidth. Then, the closely spaced AMC reflector is employed under the DB-DPWFA for performance improvement including unidirectional radiation, low profile, gain enhancement, and higher front-to-back (F/B) ratio. The final antenna design exhibits an 8% and 13% impedance bandwidths for 2.45 GHz and 5.8 GHz frequency regions, respectively. The overall gain enhancement of about 4 dB is achieved. The F/B ratio is approximate to 20 dB with a 16 dB improvement. The measured results are inconsistent with the numerical values. The presented design is a suitable candidate for radio frequency identification (RFID) reader application.