Cellulose was dissolved in an aqueous solution of sodium hydroxide (NaOH) and urea followed by the addition of barium sulfate (BaSO ₄ ) to yield the BaSO ₄ /cellulose composite particles. The morphology, particle size, and BaSO ₄ content of the composite particles were adjusted by controlling the feed ratio of cellulose and BaSO ₄ . The cellulose within the composite particles then reacted with 3,5-dimethylphenyl isocyanate. The resulting materials were utilized as the chiral stationary phases (CSPs) whose enantioseparation capabilities were evaluated by various chiral analytes. Due to the mechanical enhancement effect of BaSO ₄ , the composite particles could be applied to the chromatographic packing materials.