摘要：Salmonella bongori, which belongs to the genus of Salmonella, is a gram-negative,
rod-shaped bacterium, typically considered a microbe of cold-blooded animals and
causes salmonellosis in human being characterized by cramping and diarrhea. These
bacteria become resistant to antibiotics and make some problems in the treatment.
Researches needed to find a more appropriate and accurate way to get help from
chitosan nanoparticle technology. Therefore, the aim of this study was to evaluate the
antibacterial effect of chitosan nanoparticles against Salmonella bongori. After
culture and preparation of Salmonella bongori, ionic gelation method was used to
produce chitosan nanoparticles. Hole plate and tube dilution methods were used in
qualitative determination of antimicrobial activities against Salmonella bongori.
Finally, zeta's analysis assay, dynamic optical scanning, and electron microscopy
performed to evaluate nanoparticles. Low molecular weight chitosan nanoparticles
were produced by analyzing the results of optical dynamics scattering (111.7 nm), zeta
analysis (20.8 mV) and microscopy (<200 nm). The diameter of the non-growth halo
at different concentrations revealed chitosan and antibiotic nanoparticles had a high
and effective effect against bacteria. According to the results, there was a significant
relationship between the chitosan nanoparticles resistance and antibiotics against
bacteria. On the other hand, the nanoparticles antibacterial properties were higher than
antibiotics, which can be deduced from chitosan nanoparticles for controlling diseases
and destroying resistant bacterial species.
