期刊名称：International Journal of Fundamental and Applied Sciences
印刷版ISSN：2278-1404
出版年度：2018
卷号：7
期号：3
页码：14-18
出版社：BioMedAsia
摘要：Background: The In-silico studies encourages design of the drug molecules, understanding and development of the
pathways, protein-protein interactions, stability of the molecules etc. these studies also help in identifying the
disorders and in turn to derive the new drug molecules. Galactosemia is one of the metabolic disorders that affect the
metabolism of the sugar galactose. It leads to accumulation of the galactose in the blood leading to quite morbidity
and genetic disorders. Objective: The current paper discuss about the interactions between the variants of Galactose-1
-phosphate uridylyltransferase (GALT). Methodology: Using the Mutater software, the mutations N314D, S135L
K285N and Q188R were incorporated in the sequence of the unmutated GALT. Results: The results obtained from
webserver PROVEAN classified the mutation N314D as neutral compared to the other three, which were deleterious.
The docking studies on Autodock Vina revealed that some mutated forms did not show 2-D interaction diagrams
despite having a good bonding interaction. It was found that the mutated forms and unmutated forms interact largely
with N314D mutated structures, which are a common incidence in galactosemia disorders.
