期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2018
卷号：25
期号：2
页码：1-6
DOI：10.26444/aaem/90535
出版社：Institute of Agricultural Medicine in Lublin
摘要：Introduction. Dobrava-Belgrade virus (DOBV) is one of the emerging pathogens which have been reported during the
last decades in Europe and have attracted the attention of researchers. The course of infection among humans may have
a varied course – from the completely asymptomatic to the more severe forms, such as haemorrhagic fever with renal
syndrome (HFRS). DOBV is hosted and carried by rodents like Apodemus flavicollis or A. agrarius, which occur commonly in
Europe.
Objective. To-date, orthohantaviruses have been reported in Poland, both in humans and animals, but detailed countryscale
studies have not yet been carried out. The aim of the study was molecular characterization of a strain which was found
in A. flavicollis in south-eastern Poland.
Materials and method. The phylogenetic analysis of the first Dobrava-Belgrade virus found in A. flavicollis in the subcarpathian
region of south-eastern Poland, presented in this study, was performed after virus proliferation in cell culture and sequencing
of specific PCR products.
Results. Based on genetic sequences of fragments of three segments (S, M and L), the isolated virus was assigned to the
Dobrava genotype, taking into consideration the most current classification of the DOBV species.
Conclusions. The Dobrava-Belgrade virus strain isolated from A. flavicollis in the subcarpathian region of south-eastern
Poland, has been molecularly characterized and assigned to Dobrava genotype, thereby the occurrence of that genotype
in Poland has been confirmed by molecular techniques.
