标题：Effect of povidone iodine, chlorhexidine digluconate and toyocamycin on amphizoic amoebic strains, infectious agents of Acanthamoeba keratitis – a growing threat to human health worldwide
期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2018
卷号：25
期号：4
页码：1-7
DOI：10.26444/aaem/99683
出版社：Institute of Agricultural Medicine in Lublin
摘要：Introduction. Free-living amoebae, ubiquitous in outer environments, in predisposing circumstances may exist as parasites,
infectious agents of Acanthamoeba keratitis. In recent decades, the vision-threatening corneal infection is a growing human
health threat worldwide, including Poland. The applied therapy is often ineffective due to diagnostic mistakes, various
pathogenicity of Acanthamoeba strains and high resistance of cysts to drugs; many agents with possible anti-amoebic
activity are still being tested. In the presented study, selected chemicals are investigated in terms of their in vitro effect on
corneal and environmental Acanthamoeba strains.
Materials and method. Samples of a corneal isolate from a patient with severe Acanthamoeba keratitis,of assessed on the
basis of genotype associations of 18S rRNA and the type strain, Acanthamoeba castellanii Neff cultivated in bacteria-free
condition, were exposed to povidone iodine, chlorhexidine digluconate or toyocamycin. In vitro population dynamics of
the strains were monitored and compared to those of control cultures.
Results. All chemicals showed anti-amoebic effects with different degrees of effectiveness. Significant differences were
observed in the in vitro population dynamics, and the morpho-physiological status of A. castellanii Neff T4 and corneal strains
determined as A. polyphaga T4 genotype, exposed to povidone iodine or toyocamycin, in comparison with chlorhexidine
taken as reference.
Conclusions. Time-dependent amoebstatic in vitro effects were demonstrated for all agents, in particular, the results of
assays with povidone iodine are promising. No significant stimulation of encystation appeared; however, as cysticidal
efficacy of chemicals is expected, complementary research is needed on different Acanthamoeba strains with modified
agent concentrations and method application.
