期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2018
卷号：25
期号：4
页码：1-4
DOI：10.26444/aaem/78696
出版社：Institute of Agricultural Medicine in Lublin
摘要：Introduction. Alcohol consumption is the world’s third largest risk factor for disease and disability. According to the
WHO report from 2011: 71% of urban respondents ty and 77% of rural respondents admit to alcohol consumption]. Lower
socio-economic status and educational levels result in a greater risk of alcohol-related injury, disease and death. Alcohol
is a common component of many medicines, as well as an ingredient in many oral hygiene home products. Mouthwashes
containing alcohol are considered to inhibit wound healing in the oral cavity. Due to the fact that many different results
are described for different concentrations of alcohol at different times, an attemptwas made to visualise the direct impact
of 7.2% and 22% alcohol on human gingival fibroblasts.
Materials and method. PANsystem 2000 was used for visualisation of the reaction of human gingival fibroblasts isolated
from gingiva on ethanol in 2 different concentrations. PANsys 3000 is a multi-system fully-automated cell culture device
used for in vitro culture and to study a variety of cell lines under conditions similar to in vivo. Observations were carried out
for 48 hours since alcohol addition. Pictures were taken in a continuous process at 5 minute intervalds and combined into
a film.
Results. Both contamination of 7.2% and 22% ethyl alcohol negatively affected morphology and cell proliferation. Addition
of ethanol at a concentration 7.2% enabled cells to regain their ability to divide and recover normal morphology after 10
hours; changes caused by 22% ethanol, however, were irreversible.
Conclusions. The obtained results suggest that daily usage of 7.2% alcohol contained in mouthwashes is non-toxic for
gingival fibroblasts, and could be recommended after periodontal surgery.
