期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2018
卷号：25
期号：4
页码：1-5
DOI：10.26444/aaem/74315
出版社：Institute of Agricultural Medicine in Lublin
摘要：Physical effort can elicit differential adaptive changes in the tissues of trained versus untrained rats. Proteolytic activity in
the extracellular matrix could be engaged in such adaptation due to its influence on the elasticity of tissues. The effects were
investigated of a single physical effort on the activity of elastase, cathepsin K, and plasmin in the skeletal muscles, heart
muscles, and aortas of untrained (UT, n=30) and trained (T, n=30) rats. T rats underwent 6 weeks of endurance training. After
the last training session, T and UT rats were divided randomly into 3 subgroups. Ten rats from each group (Tpre, n=10) and
(UTpre, n=10) were sacrificed. The other 20 rats from each group performed 60 min. of aerobic exercise and were sacrificed
immediately post exercise (T0h, n=10; UT0h, n=10) or 3h later (T3h, n=10; UT3h, n=10). Enzyme activity was measured
fluorometrically. Cathepsin K and plasmin activity increased in the soleus muscles of UT0h versus UTpre, plasmin activity
increased also in UT3h versus UTpre. Elastase, cathepsin K and plasmin activity increased in the heart muscles of T0h and
T3h versus Tpre. No aortic differences were observed. Thus, a single bout of physical effort elicited different responses in
tissues of T versus UT rats. Increased proteolytic enzyme levels in muscles could influence tissue remodeling. Unchanged
aortic cathepsin K levels may help prevent aortic remodeling and neointima formation.
