期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2019
卷号：26
期号：1
页码：1-7
DOI：10.26444/aaem/82626
出版社：Institute of Agricultural Medicine in Lublin
摘要：Introduction. Mastitis may result in physical, chemical and microbiological changes in milk and pathological lesions in the
glandular tissue. Milk derived from cows with mastitis may become a cause of infections in humansw and animals.
Objectives. The aim of this study was to assess the effectiveness of selected dipping agents in the inactivation of several
bacteria that may cause mastitis in cattle.
Materials and method. Three strains of each of the following species: Escherichia coli, Klebsiella pneumoniae, Serratia
marcescens, Staphylococcus aureus and Listeria monocytogenes, isolated from milk, were used in the study. Identification
of isolates was carried out using the automatic system VITEK2 Compact. Evaluation of the genetic similarity between the
tested strains was made using the RAPD technique. Drug susceptibility of strains was evaluated with the disc diffusion
method. Assessment of the effectiveness of iodine, stabilized iodine, povidone iodine and chlorhexidine was performed
using fragments of skin from cow teats.
Results. All the tested strains were genetically different. Most of them were susceptible to the studied antibiotics. Only two
strains of L. monocytogenes were resistant to all the studied antibiotics. The percentage rate of reduction in the number of
bacteria after using of dipping agents was very high (>90%). The most susceptible to the dipping preparations used were
L. monocytogenes (99.6 – 99.9%). Stabilized iodine was the most effective dipping agent for all tested bacteria, causing a
reduction rate in the number of bacteria from 99.80% (E. coli) – 99.99% (S. aureus, L. monocytogenes).
Conclusions. The results obtained may contribute to a reduction in udder infections in cows, especially mastitis, and
improve the quality of the milk.
