标题：Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains
期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2019
卷号：26
期号：1
页码：1-5
DOI：10.26444/aaem/105394
出版社：Institute of Agricultural Medicine in Lublin
摘要：Introduction. Various Acanthamoeba species are free-living organisms widely distributed in the human environment.
Amphizoic amoebae as facultative parasites may cause vision-threatening eye disease – Acanthamoeba keratitis, mostly
among contact lens wearers. As the number of cases is increasing, and applied therapy often unsuccessful, proper hygienic
measures and effective contact lenses disinfection are crucial for the prevention of this disease. Available contact lens solutions
are not fully effective against amphizoic amoebae; there is a need to enhance their disinfecting activity to prevent amoebic
infections. The use of developing nanotechnology methods already applied with success in the prevention, diagnostic and
therapy of other infectious diseases might be helpful regarding amoebic keratitis. This study assesses the in vitro effect of
selected contact lens solutions conjugated with nanoparticles against Acanthamoeba trophozoites.
Materials and method. Three selected contact lens solutions conjugated with silver and gold nanoparticles in concentration
of 0.25–2.5 ppm were used in vitro against the axenically cultured ATCC 30010 type Acanthamoeba castellanii strain. The
anti-amoebic efficacy was examined based on the oxido-reduction of AlamarBlue. The cytotoxicity tests based on the
measurement of lactate dehydrogenase (LDH) activity were performed using a fibroblast HS-5 cell line.
Results. Enhancement of the anti-amoebic activity of contact lens solutions conjugated with selected nanoparticles
expressed in the dose dependent amoebic growth inhibition with a low cytotoxicity profile was observed.
Conclusions. Results of the study showed that conjugation of selected contact lens solutions with silver nanoparticles
might be a promising approach to prevent Acanthamoeba keratitis among contact lens users.
