期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2019
卷号：26
期号：3
页码：1-4
DOI：10.26444/aaem/99669
出版社：Institute of Agricultural Medicine in Lublin
摘要：Existing research for using the protective antigen (PA) of Bacillus anthracis as a vaccine component shows that protection
against anthrax may be obtained using fragments of this protein. The aim of the research is to check whether the selected
protein fragment of the protective antigen (domain 4) encoded by an appropriate nucleotide sequence of gene pag of
B. anthracis, was expressed in the bacterial system of E. coli. In order to examine the selected sequence of the pag gene, a
PCR reaction and a highly effective TOPO cloning strategy were used, followed by purification of the recombinant proteins
and their detection by a western-blot method. In the planning of the PA4 antigen expression a higher level of effectiveness
in production of small protein – domain 4 – was anticipated. As a result, the 139 amino acids protein fragment of B. anthracis
PA (domain 4) was isolated. The research may have found the basis for in vivo research aimed at finding potential anthrax
vaccine components.
