标题：Occurrence of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti in Ixodes ricinus ticks collected from selected areas of Opolskie Province in south-west Poland
期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2019
卷号：26
期号：4
页码：1-4
DOI：10.26444/aaem/110214
出版社：Institute of Agricultural Medicine in Lublin
摘要：Introduction. Ticks (Acari: Ixodida) are vectors and/or reservoirs of many pathogens, i.e. Borrelia burgdorferi sensu lato,
Anaplasma phagocytophilum and Babesia microti. These pathogens are ethiological agents of such diseases as Lyme
borreliosis, human granulocytic anaplasmosis and human babesiosis.
Objective. The aim of the study was to evaluate the role of the Ixodes ricinus in the transmission of Borrelia burgdorferi
sensu lato, Borrelia afzelii, Borrelia garinii, Borrelia burgdorferi sensu stricto, Anaplasma phagocytophilum and Babesia microti
in Opolskie Province in Poland.
Materials and method. DNA from 222 ticks was isolated by the ammonia method. The pair of primers specific to the
flagelline gene was used to detect of B. burgdorferi s. l. To detect of genospecies of this spirochete, three pairs of internal
primers were used. In turn, two pairs of primers specific to the 16S rDNA gene and the 18S rRNA were used, respectively, for
the detection of A. phagocytophilum and B. microti. Borrelia burgdorferi s. l., A. phagocytophilum, and B. microti were detected
in 4.5%, 2.7% and 5.4% of examined ticks, respectively.
Results and conclusions. Of the ten ticks infected with B. burgdorferi s. l., B. afzelii was found in seven, undefinied genospecies
in two, and mixed infection with B. afzelii and B. burgdorferi s. s. in one. The study demonstrated the potential risk of exposure
of humans and animals to infections of B. burgdorferi s. l., A. phagocytophilum and B. microti in the examined area of Poland.
关键词：Ixodes ricinus; Opolskie Province; Poland; Borrelia burgdorferi sensu lato; Anaplasma phagocytophilum; Babesia microti; Borrelia
burgdorferi sensu lato genospecies
