摘要：This study was carried out to identify antibiotic resistant bacteria isolated from Catfish (Clarias gariepinus) and
Tilapia (Oreochromis sp.) fish types at Eleyele, University of Ibadan fish farm and Asejire all within Ibadan metropolis.
Antibiotic susceptibility testing was carried out on Ninety (90) of the previously isolated bacterial isolates using the
agar gel diffusion technique. Antibiotics used were Gentamycin, Ofloxacin, Nitrofurantoin, Ampicillin, Augmentin,
Amoxicillin, Ceftazidime, Cefuroxime, Ciprofloxacin, Ceftriaxone, Erythromycin, Amikacin and Ticarcillin. Various
level of multi drug resistance was observed among the isolates. The bacterial population identified from this study
includes; Staphylococcus aureus, Salmonella sp., Shigella sp., Pseudomonas aeruginosa, Klebsiella pneumonia,
Klebsiella oxytoca, Klebsiella ozaenae, Acinetobacter baumanii, Proteus vulgaris, Escherichia coli, Enterobacter
aerogenes, Citrobacter freundii and Moraxella catarrhalis. Twelve (12) of the isolates were further subjected to
plasmid profile analysis in which, plasmids of different sizes were found for 10 of the isolates. It was observed that only
one Shigella sp., out of the Five (5) isolates had lost the plasmids and became responsible to the earlier resistant
antibiotics while the other four isolates Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus
maintained their resistance to the antibiotics. The spread of multidrug resistance pathogens has constituted a major
impediment to the control of infectious diseases, and since this study has shown that fish samples could be a reservoir of
bacteria carrying R-plasmids as well as genes responsible for resistance, therefore, the indiscriminate use of antibiotics
should be discouraged and also national regulations on the use of antibacterial should be introduced and enforced.
