期刊名称：Annals of the University Dunarea de Jos of Galati. Fascicle VI : Food Technology
印刷版ISSN：1843-5157
电子版ISSN：2068-259X
出版年度：2018
卷号：42
期号：2
页码：1-13
出版社：Galati University Press
摘要：Optimal conditions of total phenolic contents (TPC) and antioxidant activity (AA)
from Opuntia ficus-indica (OFI) seeds using ultrasound-assisted extraction
(USAE) and response surface methodology (RSM) were investigated. The Box–
Behnken design was used to investigate the effects of three independent variables:
acetone concentration (40-80%), ultrasonic time (5-15 min) and sonication
amplitude (40-100%). The experimental values of TPC and AA under the optimal
conditions were 4.70± 0.13 mg GAE/g DW and 2.15±0.02 mg GAE/g DW,
respectively. High Performance Liquid Chromatography analysis in optimized
conditions revealed the presence of 10 and 9 phenolic compounds respectively,
which were then identified and quantified. The effect of optimum extraction
conditions using USAE on cell surface changes of OFI seeds powder was
observed by scanning electron microscopy.
