标题：Frequency of icaA and icaD determinants and biofilm formation among coagulase-negative staphylococci associated with nasal carriage in neonatal intensive care units
出版社：European HIV/AIDS and Infectious Diseases Academy
摘要：Introduction Nasal colonization with coagulase-negative staphylococci (CoNS) may be a preliminary
risk factor for systemic infection. The aim of this study was to assess the frequency of ica A and D genes
and biofilm formation among hospital-acquired nasal colonizing CoNS strains isolated from neonates in
the neonatal intensive care units (NICUs). Antibiotic sensitivity patterns and some relevant risk factors
were estimated.
Methods This study assessed nasal colonization with CoNS among neonates at days one and three of
admission to NICUs of Beni-Suef University Hospital and Beni-Suef General Hospital from November
2015 to May 2016. The isolates were screened and identified; susceptibility testing was performed.
Biofilm formation was examined using the Congo red agar method. Isolates identified as CoNS were
tested by polymerase chain reaction (PCR) for the presence of mecA and icaA and icaD genes.
Results A total of 340 nasal swabs were collected from 170 neonates. The incidence of nasal
colonization with CoNS was 50%. The species most frequently isolated were S. haemolyticus and S.
epidermidis. Multidrug resistance (MDR) was detected in 86% of isolates. It was found that there was a
strong association between the presence of mecA gene and phenotypic resistance to methicillin and also
the presence of the icaA gene and biofilm formation.
Conclusions Neonates admitted to NICUs can become reservoirs for CoNS strains, leading to
potential dissemination of MDR strains into the community.
关键词：Coagulase;negative Staphylococcus; icaA; icaD; mecA; neonatal intensive care units
