期刊名称：Research in Physical Education, Sport and Health
印刷版ISSN：1857-8152
出版年度：2019
卷号：8
期号：2
页码：1-4
出版社：Faculty of Physical Culture, Skopje
摘要：Genetic variations of appropriate marker genes define an individual’s predisposition to sport
performance and body weight. The influence of many genes and their allelic polymorphism on sport
performance and obesity has been identified in various studies. In this study we optimised the PCR
condition for the amplification of six genes of which two are so called “sports genes”, ACE and ACTN3,
and four “obesity genes”, FTO, PPARγ, FABP2, ADRB3, respectively. DNA was isolated from blood
samples using Promega kit. PCR amplifications for all genes were performed in 25 μL final volume with
different thermal cycling conditions. One polymorphism can be identified using end point PCR (ACE gene),
while in other cases it is necessary to perform Restriction Fragment Length Polymorphism (RFLP) analysis
using appropriate restriction endonucleases. The optimisation of PCR conditions is the first step followed
by the molecular characterisation and the correlation analysis of those results with previously measured
anthropometric parameters.
关键词：genetic factors; sport genes; obesity genes; sport performance; PCR condition
