标题：Comparative effect of Pennisetum glaucum and alendronate on biochemical and histological analyses of lumbar vertebrae and liver for rats fed cafeteria diet associated with glucocorticoid therapy
期刊名称：Journal of Materials and Environmental Science
印刷版ISSN：2028-2508
出版年度：2018
卷号：9
期号：2
页码：716-723
DOI：10.26872/jmes.2018.9.2.79
出版社：University of Mohammed Premier Oujda
摘要：Glucocorticoid therapy causes serious side effects, including osteoporosis, growth delay,bone fragility, and increasing bone fractures. The purpose of this study is to evaluate theeffect of aqueous extract of Pennisetum glaucum (AEPG) and alendronate (ALN) on lumbarvertebrae in rats fed cafeteria (CAF) diet associated with methylprednisolone (MPN), aglucocorticoid widly used in therapy. Thirty six Wistar rats were randomized into six groupsof six animals each: control group received standard diet for rats; CAF group receivedcafeteria diet; CAF+MPN group received CAF+MPN (9 mg/kg body weightintraperitoneal); CAF+AEPG group received CAF+AEPG (250 mg/kg );CAF+MPN+AEPG group received (CAF, 9 mg/kg, 250 mg/kg respectively);CAF+MPN+ALN group (CAF, 9 mg/kg, 2 mg/kg respectively). After six weeks oftreatment, animals were weighted and sacrificed. Lumber (L1) mineral content was assessed.Lumbar (L2-3) and liver serial sections of 5 μm thicknesses were cut for pathology. Our datashowed that calcium and phosphorus content decreased in MPN groups. AEPG, CAF andALN seem to correct glucocorticoid therapy phosphocalcic disorders. We found significanthistopathological alterations signing a hepato-steatosis in both CAF and CAF+MPN groups;alterations healed by AEPG. The groups receiving CAF and MPN show bone loss, anddecreased lumbar trabecular bone. In our research CAF+MPN+ALN andCAF+MPN+AEPG groups showed a greater improvement of lumbar trabecular bone. AEPGdemonstrated, as well as ALN, a beneficial effect on bone fragility induced bymethylprednisolone treatment. Pennisetum glaucum, an ethnopharmacological plant, may beused or associated in osteoporosis treatment and bone fragility prevention.
关键词：Pennisetum glaucum; lumbar vertebrae; cafeteria diet; Glucocorticoid; Alendronate; rat
