期刊名称：Journal of Materials and Environmental Science
印刷版ISSN：2028-2508
出版年度：2017
卷号：8
期号：s
页码：4923-4933
出版社：University of Mohammed Premier Oujda
摘要：The aim of this study is to investigate in vitro anticancer, antioxidant and antimicrobial
activities of the crude extract of Lyngbya aestuarii, a marine filamentous non-heterocystous
cyanobacterium frequently found in the rocky shores of the Moroccan Atlantic Ocean, where
it forms epilithic hairy mat-like biomasses in the uppermost part of intertidal zone. The
species was first taxonomically identified based on a combined phenotypic attributes and
molecular approach using the partial sequencing of 16S rRNA. The in vitro anticancer
activity of Lyngbya aestuarii extract was performed on HepG2, HT-29, T47D and MG-63
human cell lines using MTT assay. Moderate cytotoxicity was revealed within 48h of
incubation in all cells and the most pronounced responses were seen in HT29 and HepG2
cells with reduced cell viability of 61.38±3.26 and 62.78±2.13%, respectively. The significant
antioxidant activity demonstrated by DPPH• radical scavenging assay (EC50=213.95 µg mL-1
)
and ferrous ion chelating ability (EC50=219.76 µg mL-1
) implies the presence of various
potent antiradicals presumed as an adaptation strategy of the species to harsh environmental
conditions in the upper tidal limit. The cyanobacterial extract screened for antimicrobial
activity using agar disc diffusion method, exhibited moderate to good antibacterial activity
against three gram positive microorganisms (Staphylococcus aureus, Bacillus subtilis,
Micrococcus luteus) and the gram negative bacterium Pseudomonas aeruginosa, as well as a
moderate inhibition towards two tested fungal species Candida albicans and Candida
parapsilosis. This study suggests that Lyngbya aestuarii may be a potent source of bioactive
molecules and further research will focus on the separation and identification of metabolites
responsible for these anticancer, antioxidant and antimicrobial activities.
