摘要：Chestnut bee pollen is a high antioxidative natural bee product. In this study the phenolic compounds in
chestnut bee pollen were determined by HPLC-DAD system. Ethanol, water and methanol were used for
the extraction of antioxidant compounds from chestnut bee pollen. The total phenolic contents and
antioxidant capacities of extracts were determined by Folin-Ciocalteu, CHROMAC (Cr(VI) reduction
antioxidant capacity) and FRAP (ferric reducing antioxidant power) methods, respectively. When compared
the amounts of phenolic compounds and spectroscopic results (total phenolic contents and antioxidant
capacities), the most suitable solvent was found ethanol for the extraction of antioxidant compounds from
bee pollen. The major phenolic compounds in the chestnut bee pollen were determined as pinocembrin,
chrysin, galangin and hyperoside with the contents of 1.246, 0.332, 0.122 and 0.516 mg/g of bee pollen,
respectively. And also it was determined that the chestnut bee pollen was inhibited 11% of DNA oxidation
in Fenton medium.
关键词：Chestnut bee pollen; HPLC; CHROMAC; DNA oxidation; phenolic compounds
