期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2019
卷号：65
期号：6
页码：491-497
DOI：10.3177/jnsv.65.491
出版社：Center for Academic Publications Japan
摘要：Exercise can improve obesity and metabolic disorders in mice fed a high-fat diet (HFD), but the role of AMPKα2 in the process remains unclear. The aim of this study was to investigate the role of AMPKα2 in the exercise-induced improvements in glucose tolerance and metabolic turnover in obesity mice. Male wild-type mice ( n =12) and AMPKα2 knockout (AMPKα2 KO) mice ( n =12) were fed a HFD for 16 wk and were then randomly divided into four groups: WT HFD group (WT HF), AMPKα2 KO HFD group (AMPKα2 KO HF), WT HFD exercise group (WT HE), and AMPK HFD exercise group (AMPKα2 KO HE). The HF groups continue to be fed a HFD from 16 wk to 24 wk, and the HE groups were fed a HFD and performed exercise training. After 8 wk of exercise, all mice were placed in an energy metabolism chamber to test their metabolic turnover, include locomotor activity, food intake, oxygen consumption (VO 2 ), carbon dioxide production (VCO 2 ), energy expenditure (EE) and respiratory exchange ratio (RER), over a period of 3 d. Exercise improved glucose tolerance, VO 2 , VCO 2 and EE in mice fed a HFD ( p <0.05). The VO 2 , VCO 2 and EE in AMPKα2 KO HE group were lower than these in WT HE group ( p <0.05). Our findings revealed exercise improved glucose tolerance and metabolic disorders in C57 and AMPKα2 KO mice fed a HFD. AMPKα2 is not essential for exercise-induced improvements in glucose tolerance and metabolic disorders..
关键词：AMPKα2;exercise;high;fat feeding;metabolic disorders;energy expenditure;respiratory exchange ratio;oxygen consumption;carbon dioxide production
