摘要：Studies on the nutritional status of vegetarians in Spain are lacking. Prevention of vitamin B 12 deficiency is the main concern, as dietary sources are of animal origin. The present study aimed to evaluate vitamin B 12 and folate status of Spanish vegetarians using classical markers and functional markers. Participants were adult and healthy lacto-ovo vegetarians (forty-nine subjects) and vegans (fifty-four subjects) who underwent blood analyses and completed a FFQ. Serum vitamin B 12 , homocysteine (Hcy), methylmalonic acid (MMA), erythrocyte folate and haematological parameters were determined. The effects of the type of plant-based diet, and the intake of supplements and foods were studied by a FFQ. Mean erythrocyte folate was 1704 ( sd 609) nmol/l. Clinical or subclinical vitamin B 12 deficiency was detected in 11 % of the subjects (MMA>271 nmol/l) and 33 % of the participants showed hyperhomocysteinaemia (Hcy>15 µ mol/l). Regarding plant-based diet type, significantly higher Hcy was observed in lacto-ovo vegetarians compared with vegans ( P = 0·019). Moreover, use of vitamin B 12 supplements involved an improvement of vitamin B 12 status but further increase in erythrocyte folate ( P = 0·024). Consumption of yoghurts was weakly associated with serum vitamin B 12 adequacy ( P = 0·049) and that of eggs with lower Hcy ( P = 0·030). In conclusion, Spanish vegetarians present high folate status but vitamin B 12 subclinical deficiency was demonstrated using functional markers. The lack of influence of dietary sources on functional markers and the strong effect of vitamin B 12 supplement intake emphasise the need of cobalamin supplementation in both lacto-ovo vegetarians and vegans.
