出版社：International Medical Journal Management and Indexing System
摘要：Cystic ovarian follicles “COF” is a common ovarian disorder diagnosed in dairy cattle that
cause significant economic losses in dairy industry. This study was designed to evaluate
efficacy of different therapeutic protocols of COF without determining cyst type. The
study was conducted on 104 Holstein-Friesian cystic cows that were divided randomly to
the following treatments: Experiment Ι: 1) Group G, n=27, subdivided into A) n=13:
treated with 20μg Buserelin-GnRH agonist and B) n=14: treated with 100μg GonadorelinGnRH agonist; 2) Group GP, n=23: received 20μg Buserelin on day 0- 500μg of PGF2α
on day 10; 3) Group GGP, n=27: received 2 doses of 20μg Buserelin 7 ds apart followed
by 500μg PGF2α 7ds from the 2nd Buserelin injection; 4) Group GPG (Ovsynch), n=17:
received 20μg Buserelin on day 0- 500μg PGF2α on day 7, and 48hrs later, 20μg Buserelin
was injected; 5) Group C, n=10: no treatment received. 22 normal cycling cows were kept
as a normal control-NC. Experiment ΙΙ: This part of the study was conducted on 34 cystic
animals that did not respond to one of the previous treatments in experiment Ι and were
divided into 2 groups: 1) Group CGP, n=19: CIDR+100μg Gonadorelin- (d 0), then 500μg
of PGF2α on day 7, insert was removed on day 9; 2) Group PI, n=15: treated by oral
administration of 5gm Potassium iodide dissolved in 500 ml sterile water for 7 successive
days. Treated Cows were observed for 35 days from the end of treatment, females
exhibited estrus were inseminated within 12 hours of detected heat. Pregnancy was
confirmed by per rectum on day 50 post-insemination. Results showed that GP protocol
achieved highest (P<0.05) estrus induction rate (EIR) recorded at 87%, followed by 70%
for G and GGP group, and lowest (53%) obtained in GPG treated cows. Conception rate at
1
st estrus and total conception rate (3 cycles after treatment) did not differ significantly
among treatment groups, while pregnancy rates (PR) were significantly different (P<0.05)
with highest (65.21%) obtained in GP protocol. Other groups recorded 63%, 55.55%, and
41.17% PR for G, GGP, and GPG respectively. In experiment ΙΙ, EIR was 47.36% and
33.33% for CGP and PI respectively with no significant difference, all conceived after 3
estrous cycles by the end of the experiment. In conclusion, GnRH+ PGF2α 10ds later was
the most suitable treatment of COF regardless of cyst type, GGP protocol might be a
profitable alternative to Ovsynch for treatment of cystic ovaries. Persistent cases of COF
could be recruited to avoid culling for other treatment options such as CIDR or Potassium
iodide with equal therapeutic efficacy.
