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  • 标题:Molecular Characterization of Escherichia Coli Isolated from Poultry Meat and its Products -
  • 本地全文:下载
  • 作者:Zakaria H. Elbayoumi ; Reyad R. Shawish ; Mohamed Hamada
  • 期刊名称:Alexandria Journal of Veterinary Sciences
  • 印刷版ISSN:1110-2047
  • 电子版ISSN:1110-2047
  • 出版年度:2018
  • 卷号:56
  • 期号:2
  • 页码:39-47
  • DOI:10.5455/ajvs.289559
  • 出版社:International Medical Journal Management and Indexing System
  • 摘要:This study was conducted on chicken breast, chicken thigh, Shish Taouk, chicken shawarma, chicken nuggets and chicken luncheon (35 of each) collected from different supermarkets at Menoufia Governorates for isolation and identification of E. coli and using PCR for detection of virulence gene. The obtained results indicated that the incidence of E. coli was 14.3%, 20%, 14.3%, 17.1%, 14.3% and 20% of examined samples of chicken breast, chicken thigh, Shish Taouk, chicken shawarma, chicken nuggets and chicken luncheon, respectively. Moreover, the incidence of serologically identified E. coli O78, O26: H11, O2: H6, O91:H21, O44:H18, O128:H2, O1:H7, O103:H2, O127:H4, O114:H4 and O158. PCR results showed shiga toxin 2 gene(stx2) detected in (O1), (O2), (O114) & (O128), while shiga toxin 1 gene(stx1) detected in (O44), (O127) & (O158), also (O78) & (O91) Positive E. coli strains for stx1 and stx2 genes, and(O26) & (O103) Positive E. coli strains for stx1, stx2 and eaeA genes.
  • 关键词:E;Coli; poultry products; PCR; virulence Gene
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