首页    期刊浏览 2024年08月31日 星期六
登录注册

文章基本信息

  • 标题:High-Throughput Automated Microscopy of Circulating Tumor Cells
  • 本地全文:下载
  • 作者:Carlos Aguilar-Avelar ; Brenda Soto-García ; Diana Aráiz-Hernández
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2019
  • 卷号:9
  • 期号:1
  • 页码:1-9
  • DOI:10.1038/s41598-019-50241-w
  • 出版社:Springer Nature
  • 摘要:Circulating tumor cells (CTCs) have the potential of becoming the gold standard marker for cancer diagnosis, prognosis and monitoring. However, current methods for its isolation and characterization suffer from equipment variability and human operator error that hinder its widespread use. Here we report the design and construction of a fully automated high-throughput fluorescence microscope that enables the imaging and classification of cancer cells that were labeled by immunostaining procedures. An excellent agreement between our machine vision-based approach and a state-of-the-art microscopy equipment was achieved. Our integral approach provides a path for operator-free and robust analysis of cancer cells as a standard clinical practice.
国家哲学社会科学文献中心版权所有