首页    期刊浏览 2024年09月18日 星期三
登录注册

文章基本信息

  • 标题:Development of a Turn-On Fluorescent Traceable Linker Employing N-Sulfanylethylcoumarinyl Amide for the Enrichment and Visualization of Target Proteins
  • 本地全文:下载
  • 作者:Takuya Morisaki ; Akira Shigenaga ; Akira Otaka
  • 期刊名称:Chemical and Pharmaceutical Bulletin
  • 印刷版ISSN:0009-2363
  • 电子版ISSN:1347-5223
  • 出版年度:2020
  • 卷号:68
  • 期号:3
  • 页码:216-219
  • DOI:10.1248/cpb.c19-00726
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:A turn-on fluorescent traceable linker based on N -sulfanylethylcoumarinyl amide (SECmide) has been developed as an advanced cleavable linker. It was successfully employed for the enrichment and selective visualization of a target protein in cell lysate. The results demonstrated that the SECmide-based traceable linker is potentially applicable to the identification of low molecular weight target proteins, a factor which has been problematic for a previously developed N -sulfanylethylanilide-based traceable linker..
  • 关键词:cleavable linker;N;sulfanylethylanilide;N;sulfanylethylcoumarinyl amide;target protein;traceable linker;turn;on fluorescent
国家哲学社会科学文献中心版权所有