期刊名称：Computational and Structural Biotechnology Journal
印刷版ISSN：2001-0370
出版年度：2020
卷号：18
页码：791-804
DOI：10.1016/j.csbj.2020.03.021
出版社：Computational and Structural Biotechnology Journal
摘要：Transposon insertion sequencing methods such as Tn-seq revolutionized microbiology by allowing the identification of genomic loci that are critical for viability in a specific environment on a genome-wide scale. While powerful, transposon insertion sequencing suffers from limited reproducibility when different analysis methods are compared. From the perspective of population biology, this may be explained by changes in mutant frequency due to chance (drift) rather than differential fitness (selection)..
关键词：Tn;seq ; Transposon insertion sequencing ; Population biology ; Random birth;death process ; Multinomial random sampling ; Bottleneck ; Distribution of fitness effects ; DFE ; Drift ; Selection
