期刊名称：Journal of Materials and Environmental Science
印刷版ISSN：2028-2508
出版年度：2020
卷号：11
期号：4
页码：647-657
出版社：University of Mohammed Premier Oujda
摘要：In order to overcome endogenous in vitro contamination, we have studied the effect ofessential oils as an alternative to conventional chemical treatments. The objective of thisstudy is to enhance the use of essential oils to inhibit the development of these fungi. Theessential oils extracted from four Moroccan aromatic plants, Oregano - three chemotypes(Origanum compactum), Thyme (Thymus vulgaris L.), Eucalyptus (Eucalyptuscamaldulensis) and Fenugreek (Trigonella foenum graecum L.) were use. The antifungalactivity of these essential oils was studied against two fungal strains Cylindrocarpon sppand Verticillium spp identified from Calodendrum capense in vitro plants. The resultsshowed a significant inhibitory effect on both fungi. This activity is manifested by the totalinhibition of mycelial growth, with a low concentration of essential oils. The best inhibitoryeffect was obtained by the use of Oregano (MIC-0.15 µl/ml for chemotype 3, MIC-0.25µl/ml for chemotype 2 and 0.5 µl/ml for chemotype 1) and Thyme (MIC- 0.25µl/ml). Inaddition, the Eucalyptus essential oil showed an incomplete inhibition (74.37%) ofVerticilium spp. and (51.28%) of Cylindrocarpon spp. However, fenugreek extract has thelowest activity compared to the other oils. The chemical composition of these essential oilswas analyzed by mass spectrometric chromatography (GC-MS). The analysis revealed thedominance of two compounds (Thymol and Carvacrol) in the most effective oils, and canrepresent the principal active ingredient in the pathogen control. Therefore, the essentialoils of Oregano and Thyme or their major compounds could be investigated to solve theproblem of contamination during in vitro culture.
