标题：COMPARING THE EFFICACY OF DISHWASH SOLUTION, DILUTED LEMON WATER, COCONUT OIL AND XYLENE AS DEPARAFFINIZING AGENTS FOR HEMATOXYLIN AND EOSIN STAINING PROCEDURE
期刊名称：International Journal of Anatomy and Research
印刷版ISSN：2321-4287
出版年度：2018
卷号：6
期号：2
页码：5176-5180
DOI：10.16965/ijar.2018.149
出版社：International Journals of Medical and Health Research
摘要：Aim: To compare the efficacy of dish wash solution, diluted lemon water, coconut oil and xylene as deparaffinizingagents for hematoxylin and eosin staining procedure.Objective: The objective is to find eco-friendly deparaffinizing agents like dish wash solution, diluted lemonwater and coconut oil as substitute to xylene and comparing the staining characteristics of each individualdeparaffinizing agent with Xylene.Materials and Methods: The study comprised of paraffin embedded 45 blocks of various tissues. Each block offour sections of 5 microns thickness was prepared. They were considered in four different groups like A, B, C andD. Tissue sections in Group A were stained with H & E method where xylene was used as deparaffinizing agent. Theother three sections were stained with H & E where dish wash solution, diluted lemon water and coconut oil wereused as deparaffinising agent’s alternative to Xylene. Staining characteristics were compared with xylene andscoring was given. The total score of 3–5 was regarded as satisfactory for diagnosis and less than that isinsufficient for diagnosis.Statitistical Analysis: Chi square test was used.Results: Adequacy of staining characteristics such as nuclear, cytoplasm, uniformity, clarity and crispiness ofstaining for diagnosis was greater with dish wash solution followed by diluted lemon water, coconut oil andxylene.Conclusion: The Eco-Friendly deparaffinizing agents such as dish wash solution, diluted lemon water, and coconutoil can be used as alternatives to xylene.
关键词：Eco;friendly Xylene Substitutes; Xylene Free Deparaffinization; Hematoxylin and Eosin Staining;Procedure
