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  • 标题:Performance of extraction methods for extracellular DNA from sediments across marine habitats
  • 本地全文:下载
  • 作者:Nathan R. Geraldi ; Rubén Díaz‐Rúa ; Lauren A. Shea
  • 期刊名称:Environmental DNA
  • 电子版ISSN:2637-4943
  • 出版年度:2020
  • 卷号:2
  • 期号:1
  • 页码:91-98
  • DOI:10.1002/edn3.48
  • 出版社:Wiley
  • 摘要:Background Using environmental DNA (eDNA) to detect taxa is quickly becoming an important technique to determine the distribution and diversity of macroorganisms. Assessing differences in communities based on eDNA extracted from sediment could enhance our understanding of marine communities among the many different marine habitats both from the present and from the past using sediment cores. Although the effect of extraction methods on metabarcoding results has been tested for both water and sediment samples, less is known about how sediment types may affect the extraction and sequencing of eDNA.
  • 关键词:18S rRNA;community ecology;conservation genetics;environmental DNA;sediment
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