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  • 标题:Non contiguous-finished genome sequence and description of Microbacterium gorillae sp. nov.
  • 本地全文:下载
  • 作者:Linda Hadjadj ; Jaishriram Rathored ; Mamadou Bhoye Keita
  • 期刊名称:Environmental Microbiome
  • 印刷版ISSN:2524-6372
  • 出版年度:2016
  • 卷号:11
  • 期号:1
  • 页码:1-9
  • DOI:10.1186/s40793-016-0152-z
  • 摘要:Strain G3T (CSUR P207 = DSM 26203) was isolated from the fecal sample of a wild gorilla (Gorilla gorilla subsp gorilla) from Cameroon. It is a Gram-positive, facultative anaerobic short rod. This strain exhibits a 16S rRNA sequence similarity of 98.2 % with Microbacterium thalassium, the closest validly published Microbacterium species and member of the family Microbacteriaceae. Moreover, it shows a low MALDI-TOF-MS score (1.1 to 1.3) that does not allow any identification. Thus, it is likely that this strain represents a new species. Here we describe the phenotypic features of this organism, the complete genome sequence and annotation. The 3,692,770 bp long genome (one chromosome but no plasmid) contains 3,505 protein-coding and 61 RNA genes, including 4 rRNA genes. In addition, digital DNA-DNA hybridization values for the genome of the strain G3T against the closest Microbacterium genomes range between 19.7 to 20.5, once again confirming its new status as a new species. On the basis of these polyphasic data, consisting of phenotypic and genomic analyses, we propose the creation of Microbacterium gorillae sp. nov. that contains the strain G3T.
  • 关键词:Microbacterium gorillae;Genome;Culturomics;Taxonomo-genomics;Gorilla stool sample
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