摘要：The aim of this study was to examine some potential effects of 4-Nonylphenol (4-NP),Bisphenol A (BPA),and mixture of them on common carp, Cyprinus carpio and demonstrate induction of vitellogenin (Vtg) as a biomarker for screening aquatic ecosystems.These compounds with other estrogenic chemicals may be responsible for disrupting effects observed in fish.A total of 100 juvenile carp were treated experimentally with 17β-estradiol (E2),and increasing doses of 4-NP,BPA and also mixture of them.Then the plasma Vtg levels were measured using indirect competitive ELISA.Results showed a significant (P<0.05) increase in Vtg levels of exposed carps with E2,maximum 4-NP,BPA and their mixture.The group treated with E2 showed high induction (490%) with respect to controls.The groups treated with maximum dose of 4-NP and BPA showed also induction of Vtg,whereas the group treated with their mixture (3 mg kg−1 b.w.4-NP and 0.01 mg kg−1 b.w.BPA) showed the highest induction (2126%).Finally,results showed that mixture of 4-NP and BPA exhibited stronger (synergistic) effects.So that,large scale monitoring of estrogenic effects caused by contamination may be considered as a biomarker in urban and industrial effluents.
关键词：Vitellogenin (Vtg);Cyprinus carpio;17β-Esteradiol (E2);4-Nonylphenol(4-NP);Bisphenol A (BPA);ELISA
