期刊名称：Pakistan Journal of Analytical & Environmental Chemistry
印刷版ISSN：1996-918X
电子版ISSN：2221-5255
出版年度：2004
卷号：5
期号：1
页码：38-41
语种：English
出版社：National Centre of Excellence in Analytical Chemsitry
摘要：The crude extract of mutarotase having 5.491 U/mL activity (1.264 U/mg specific activity) was subjected to sephadex G-150 column and obtained 16.518 U/mg specific activity. Optimum pH and temperature were 7.5 and 40 0C having maximum activity of 4.273 U/mL and 4.918 U/mL respectively. It was noted that,200 mg substrate concentration showed highest activity 5.607 U/mL and that of 5.519 U/mL at 500 L enzyme concentration. The study on effect of stabilizers showed that glycerol (15 %) as best one among sodium benzoate and sodium citrate with the activity of 3.71 U/mL even after 30 days..
