期刊名称：Pakistan Journal of Analytical & Environmental Chemistry
印刷版ISSN：1996-918X
电子版ISSN：2221-5255
出版年度：2009
卷号：10
期号：1
页码：25-33
语种：English
出版社：National Centre of Excellence in Analytical Chemsitry
摘要：The maximum ethanol production and β-fructofuranocidase formation under fermentation studies were carried out in microprocessor controlled 23-L stainless steel fermenter at the following conditions: Temp = 40 ˚C (wild &mutant organism),pH= 5.5,carbon source (molasses=15% sugar) nitrogen source (ammonium sulphate 0.75%),300 RPM stirring speed and oxygen flow rate was 0.1 vvm. Mutant. Strain of thermotolerant Kluyveromyces marxianus M15 produced maximum production of ethanol at 48hr.All Kinetic Parameters have been studied for the utilization of substrate and production of maximum ethanol for both wild and mutant strains. It has been observed that wild strain was growing up to 550C while the mutant strain was growing up to 650C. In this comparison study,wild and mutant strain showed that Mutant-derived M15 was stronger over its parental culture due to its more thermal stability and production of ethanol at 65 oC at which wild organism could not grow..
