期刊名称：Pakistan Journal of Analytical & Environmental Chemistry
印刷版ISSN：1996-918X
电子版ISSN：2221-5255
出版年度：2011
卷号：12
期号：1
页码：49-54
语种：English
出版社：National Centre of Excellence in Analytical Chemsitry
摘要：A rapid and reliable analytical method based on high-performance liquid chromatography (HPLC) with UV detection (221nm) has been developed for the determination of the anti-hyperglycemic agent Pioglitazone in pharmaceutical formulations and biological fluids (serum and urine) after clean-up with solid-phase extraction. Chromatographic separation was achieved with a Chromolith Æ Performance RP-18e (100×4.6mm) column using mobile phase composition of acetonitrile: mixed phosphate buffer (pH 2.5;10mM) (30:70,v/v) with a flow rate of 2.0mL/min. The total run time was 2 min. under optimized conditions. The calibration curve was found to be linear in the range of 1-10 µg mL -1 with regression coefficient of 0.9996,and the lower limit of detection 72 ng/20µL injection. The method has been validated for the system suitability,linearity,precision and accuracy,limits of detection,specificity,stability and robustness. The %recovery of Pioglitazone in pharmaceutical formulations was found to be 104.7%. The assay has been applied successfully to the pharmaceutical Tablet samples and biological fluids (serum and urine) of healthy volunteers..
