摘要:Stichopus horrens is one of the sea cucumber species that has the potential to be developed as a source of antibacterial from the sea. Samples of the sea cucumber S. horrens used in this study collected from the Ratai bay waters, Lampung in April 2016. This study aims to determine the type of secondary metabolites, antibacterial activity and compound composition containing in the methanol extract of sea cucumber S. horrens. Extraction method used in this study was the maceration method using methanol. Identification of secondary metabolites by observation of color reactions, precipitation, and foam. The method used to antibacterial activity test was the agar diffusion method, while identification of the composition of compounds performed with Gas Chromatography-Mass Spectroscopy (GC-MS) method. The results showed that the type of secondary metabolites contained in the extract of sea cucumber S.horrens were saponin and terpenoid. The methanol extract of sea cucumber S. horrens showed antibacterial activity against all bacterial test. Based on result of showed 7 compounds have a similarity index same or more than 90%. Compounds consist of 9,12- octadecadienoic, hexadecanoic acid, 3,8-di-tert-butyl-1,10-phenanthroline, 1,13-tetradecadiene and 6,9-pentadecadien-1-ol. The compound with the biggest abundance was 6,9-pentadecadien-1-ol (25.48%) and retention time was 18.597 minutes.
其他摘要:Stichopus horrens merupakan salah satu jenis teripang yang memiliki potensi untuk dikembangkan sebagai sumber bahan antibakteri dari laut. Sampel teripang S. horrens yang digunakan dalam penelitian ini berasal dari perairan Teluk Ratai Lampung pada bulan