摘要：Method of foreign gene transfer in red seaweed Kappaphycus alvarezii has been reported, however, limited number of transgenic F0 (broodstock) was obtained. This study was conducted to improve the method of gene transfer mediated by Agrobacterium tumefaciens in order to obtain high percentage of K. alvarezii transgenic. Superoxide dismutase gene from Melastoma malabatrichum (MmCu/Zn-SOD) was used as model towards increasing adaptability of K. alvarezii to environmental stress. The treatments were the culture media and recovery duration, and each treatment consisted of three replications. The best method was co-cultivation using liquid media, then recovery was conducted in liquid media for 10 days. That treatment allowed higher transformation percentage (90%), regeneration efficiency (90%), putative bud efficiency (100%), number of buds and explants sprouted (100%) and transgenic explants (100%). The transgenic explants showed an amplification PCR product of MmCu/Zn-SOD gene fragment, whereas the non-transgenic explants showed no amplification product. All results revealed that suitable method of transgenesis for K. alvarezii has been developed.
其他摘要：Metode introduksi gen asing pada rumput laut Kappaphycus alvarezii telah dilaporkan, namun jumlah transgenik F0 (induk) yang diperoleh sedikit. Penelitian ini dilakukan untuk memperbaiki metode transfer gen yang dimediasi oleh Agrobacterium tumefaciens un
