期刊名称：Biosaintifika: Journal of Biology & Biology Education
印刷版ISSN：2085-191X
电子版ISSN：2338-7610
出版年度：2010
卷号：2
期号：1
页码：53-60
DOI：10.15294/biosaintifika.v2i1.1151
语种：English
出版社：Universitas Negeri Semarang
摘要：This study aims to prove the effectiveness of zinc as a micronutrient to increase immunostimulatory Reactive Oxygen Intermediate (ROI) production in balb/c mice infected by Salmonella typhimurium. Research was designed using randomized the Post Test Only Control Group Design. The sample of 24 male mice balb/c are grouped into four groups, i.e. one control group and three treatment groups (treatment dose of 78 ppm/day; 169 ppm/day and 260 ppm/day) intraperitoneally for 14 days with each group consisting of six rats. The independent variable is the provision of zinc with various doses, dependent variable is the ROI production of macrophages, which are checked by using Nitroblue Tetrazolium (NBT) Reduction Assay, and control variables are body weight, gender and age. All mice infected with Salmonella typhimurium 105 intraperitoneally on day 8th. At the end of treatment mice were killed and was examined for levels of ROI. Inspection results were analyzed by Kruskal Wallis test. Result showed that doses of zinc of 78 ppm/day have immunostimulatory effective as the highest ROI production of mice Balb/c infected with Salmonella typhimurium.
