期刊名称：Biosaintifika: Journal of Biology & Biology Education
印刷版ISSN：2085-191X
电子版ISSN：2338-7610
出版年度：2012
卷号：4
期号：1
页码：42-46
DOI：10.15294/biosaintifika.v4i1.2267
语种：English
出版社：Universitas Negeri Semarang
摘要：In an effort to produce soybean that are resistant to pests and diseases and stress, the breeding was done by tissue culture, through callus. The use of callus is the further step in the research phases of somaclonal induction or genetic transformation. This study aimed to determine the condition of light, the optimal concentration of 2,4 D in any combinations of treatment. The research was using randomized block design consisting of two factors: the concentration of 2,4 D and the light. There was 4 levels of 2,4 D concentration (0 ppm; 3 ppm; 6 ppm; 9 ppm), and 2 levels of light exposure (24 and 0 hours). The observed variable was the time of the callus emerged, the percentage of the callus growth and the weight of the callus. The result showed that the concentration of 2,4 D was the factor affecting callus induction, whereas 2,4D and the interaction of light condition was not effective to induce callus. Based on Duncan test, the optimum concentration of 2,4 D was 9 ppm.
其他摘要：Dalam upaya menghasilkan kedelai yang tahan terhadap hama penyakit, dan cekaman, maka dilakukan pemuliaan dengan kultur jaringan, melalui kalus. Penggunaan kalus sebagai tahap penelitian selanjutnya yaitu induksi variasi somaklonal atau transformasi genet
