期刊名称：Biosaintifika: Journal of Biology & Biology Education
印刷版ISSN：2085-191X
电子版ISSN：2338-7610
出版年度：2014
卷号：6
期号：1
页码：52-59
DOI：10.15294/biosaintifika.v6i1.3785
语种：English
出版社：Universitas Negeri Semarang
摘要：In Indonesia, Carica pubescens (Lenne & K. Koch) Badillo and Vasconcellea pubescens only found in mountainous areas in Dieng and Bali. Tissue culture is an efficient propagation technique. The aim of this research was to assess the effect of the type and concentration of plant growth regulator on embryogenic callus induction on ½ MS medium in C. Pubescens. The first study (induction) was a two factor experiment were prepared using a nested plot randomized complete block design (Split block design) with 4 replications. The second study (callus regeneration) is a one-way experiment which prepared using a completely randomized design (CRD) with four replications. Data were analyzed using ANAVA and LSD to further test error level of 5%. The results showed that the induction of embryogenic callus from leaf tissue explants obtained at the highest 2,4-D treatment PGR 3 mg/l+TDZ 1 mg/l were added to the media ½ MS, produce weight percentage of callus and callus highest. For regeneration of PGR BAP with a concentration of 4 mg/l in MS medium containing activated charcoal NAA 0.2 mg/l, a greater percentage of callus formation into buds and the number of callus forming buds with optimal results. Based on the research results, it suggested to induce embryogenic callus using PGR combination with 2,4-D and thidiazuron, while for regeneration was suggested to use PGR BAP and NAA.
其他摘要：Carica pubescens (Lenne & K. Koch) Badillo atau Vasconcellea pubescens, di Indonesia hanya ditemukan di daerah pegunungan Dieng dan Bali. Kultur jaringan merupakan teknik perbanyakan yang efisien. Tujuan dari penelitian ini adalah untuk mengkaji pengaruh
