期刊名称：Biosaintifika: Journal of Biology & Biology Education
印刷版ISSN：2085-191X
电子版ISSN：2338-7610
出版年度：2014
卷号：6
期号：1
页码：67-75
DOI：10.15294/biosaintifika.v6i1.3787
语种：English
出版社：Universitas Negeri Semarang
摘要：Tiger shrimp cultivation in Indonesia has been growing rapidly. The main obstacle is the shrimp farming vibriosis disease caused by the bacterium Vibrio algynoliticus. The aim of this research was to determine the effects of neem leaf extract antibakeri against Vibrio algynoliticus. This study used a dilution method to determine the antibacterial effect of neem leaf extract against Vibrio algynoliticus bacteria in vitro. The concentration of the extract used (%): 0; 2.5; 5; 7.5; 10; 12.5 and as a control consisting of a positive control, and negative control. Data collection to determine the MIC (Minimum Inhibitory Concentration) was done by comparing the clarity of culture in TSB medium 2% on a variety of different concentrations, the positive control and a negative control. Determination MBC (Minimum Bacterisidal Concentration) was done by looking at the presence or absence and the number of colonies of bacteria Vibrio alginolyticus that appears in the subculture medium TSA 2% after 24 h incubation. The results showed that the value of MIC was 5%, as shown by the tube began to clear. Value of MBC of neem leaf extract against Vibrio alginolyticus was characterized by a concentration of 12.5% has been no emergence of bacterial colonies Vibrio alginolyticus. Based on the research, it concluded that neem leaf extract can provide antibacterial effect against bacteria Vibrio alginolyticus in vitro.
其他摘要：Budidaya udang windu di Indonesia telah berkembang pesat. Salah satu kendala budidaya udang adalah penyakit Vibriosis yang disebabkan oleh bakteri Vibrio algynoliticus. Tujuan penelitian ini adalah untuk mengetahui efek antibakeri ekstrak daun mimba terha
关键词：antibacterial; neem leaf extract; Vibrio alginolyticus
