期刊名称：Biosaintifika: Journal of Biology & Biology Education
印刷版ISSN：2085-191X
电子版ISSN：2338-7610
出版年度：2017
卷号：9
期号：1
页码：58-65
DOI：10.15294/biosaintifika.v9i1.6783
语种：English
出版社：Universitas Negeri Semarang
摘要：Botryococcus sp. is microalgae species that has high lipid content. Mutagenesis induced by Gamma 60Co irradiation can be utilized to alter Botryococcus sp. characteristics to get microalgae mutant strain that have better characteristics than the wild strain. The aim of this research was to know the effect of gamma 60Co irradiation to the growth, biomass, total lipid content and fatty acid compotition characteristics of Botryococcus sp. Botryococcus sp. was irradiated with different doses of gamma ray of 60Co (0, 2, 4, 6, and 10 Gy). Biomass and lipid content was analysed by quantitative analysis. Fatty acid composition was analyzed by Gas Chromatography-Mass Spectrometry. Results showed that Gamma irradiated gave an effect on growth, biomass and lipid content of Botryococcus sp. There was significantly different only between control (0 Gy) and 10 Gy irradiated microalgae. The highest biomass and lipid content are found in 10 Gy irradiated microalgae are 0.833 gram biomass and 41 % lipid content. Fatty acid profile of Botryococcus sp. control has 6 fatty acids while 10 Gy irradiated microalgae has 12 fatty acids, with the long-chain fatty acids increased, where as short-chain fatty acids decreased. This research could be the basis for engineering of microalgae for biodiesel production.
