期刊名称：Biosaintifika: Journal of Biology & Biology Education
印刷版ISSN：2085-191X
电子版ISSN：2338-7610
出版年度：2018
卷号：10
期号：2
页码：388-394
DOI：10.15294/biosaintifika.v10i2.14891
语种：English
出版社：Universitas Negeri Semarang
摘要：Botryococcus sp. is an important microalgae with high content of saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs) for biofuel production sources. Induction of mutagenesis using irradiation can potentially alter the characteristic of mutant strain. The aim of this study was to know the effect of electron-beam irradiation on the growth profile and fatty acid composition of Botryococcus sp. In this study, Botryococcus sp. adjusted an optical density 1.0 after exposed to different doses of electron-beam irradiation (160 kGy, 240 kGy, and 320 kGy) and induced random mutagenesis for strain improvement was observed based on high energy (1.5 MeV and 2 mA). Several mutants obtained were designated as strains of B160, B240, and B320 respectively. The profile growth was significantly different between the control (0 kGy) and irradiated microalgae strain. The highest growth was found in B320 mutant. Fatty acid of Botryococcus sp. control produced 7 fatty acids, B160 produced 7 fatty acids, B240 produced 6 fatty acids, whereas B320 produced 9 fatty acids. B320 produced hydrocarbon and phtalic acid as well as fatty acids. Total SFAs and MUFAs of B240 was increased 1.6 times compared to the wild type. The results would give some implications to improve the quality of biodiesel from Botryococcus sp.
