标题：EFFECT OF L- CARNITIN AND THERMAL STRESS ON FREE RADICAL AND ANTIOXIDANT LEVELS IN RATS DURING THE EXHAUSTIVE SWIMMING EXERCISES AT HYPOTHERMIC AND HYPERTHERMIC WATER TEMPERATURES
期刊名称：Ovidius University Annals, Series Physical Education and Sport/Science, Movement and Health
印刷版ISSN：1224-7359
出版年度：2010
卷号：10
期号：1
页码：19-28
语种：English
出版社：Ovidius University Press
摘要：Carnitine plays an important role in lipid metabolism by transporting long-chain fatty acids into the mitochondria for beta-oxidation.The aim of this study was to explain the effect of acute L-carnitine on antioxidant levels and lipid peroxidation in rats exposed to thermal stress.Eight groups (E18°C,E28°C and E38°C exhaustive swimming groups;CE18°C,CE28°C and CE38°C Lcarnitine and exhaustive swimming exercises groups;CS28°C (the sedentary group which is given carnitine);S28°C ( only the sedentary group),were formed and a total of 48 Sprague Dawley male rats,weighing 250-300 g were used in this study.In the study,the L-carnitine was given to the groups 1-1.5 hours before the exercises in the doses of 100 mg/kg by intraperitoneal (i.p.) way.Exhaustive swimming tests were made in a rectangle shaped glass water tank that was 80x60x60 cm3 in size.Uncoordinated movements and staying under the water for 10 seconds without swimming at the surface were accepted as the exhaustion criteria of the rats.In the blood samples,the SOD,GR,POD and CAT are analyzed with spectrophotometric method,and MDA is analyzed with HPLC-FLD method.In the statistical analysis of the study,Anova,Duncan and T Tests are used.According to the results of the study,the MDA level of the CE18°C group is significantly higher than those of CE28°C and CE38°C groups (p<0.05).While the GR levels of E18°C group is significantly higher than the CE18°C group (p<0.05,),the POD levels are significantly low (p<0.05,).The POD levels of CE28°C group is significantly higher than those of E28°C group (p<0.05).The findings that are gathered at the end of the study showed that the oxidative stress increases under hypothermic circumstances and it cannot prevent the free radical formation of the L-carnitine under hypothermic conditions,in addition it increases the antioxidant activity under thermal stress.The L-carnitine supply under thermal stress may show an antioxidant effect and be useful in sports activities.
