摘要：PDE4B was previously shown to be a dominant PDE4 subtype of neutrophils.However,its physiological role in the neutrophil function has not been evaluated.In this study,the inhibitory eff ects of a phosphodiesterase 4B (PDE4B)- selective inhibitor (compound A) and subtype non-selective PDE4 inhibitors (rofl umilast and cilomilast) were evaluated in human peripheral blood cells.Compound A,rofl umilast and cilomilast in a similar manner inhibited TNF-a production by LPS-stimulated human mononuclear cells.However,the inhibitory eff ect of compound A on IL-8 or LTB4-induced chemotactic response of neutrophils was modest even at the highest concentration (10 µmol L–1),whereas rofl umilast and cilomilast inhibited IL-8 or LTB4-induced neutrophil chemotaxis.Our results suggest that PDE4B does not play an important role during the chemotactic response of human neutrophils.
