摘要：The main aim of the current research was to study the effect of porphyra-334,one of mycosporine-like amino acids (MAAs),well known as UV-absorbing compounds,on UVinduced apoptosis in human immortalized keratinocyte (HaCaT) cells.Due to their UV-screening capacity and ability to prevent UV-induced DNA damage,MAAs have recently attracted considerable attention in both industry and research in pharmacology.Herein,human HaCaT cells were used to determine the biological activities of porphyra-334 by various in vitro assays,including proliferation,apoptosis and Western blot assays.The proliferation rate of UV-irradiated HaCaT cells was significantly decreased compared to the control group.Pretreatment with porphyra-334 markedly attenuated the inhibitory effect of UV and induced a dramatic decrease in the apoptotic rate.Expression of active caspase-3 protein was increased in response to UV irradiation,while caspase-3 levels were similar between cells treated with porphyra-334 and the non-irradiated control group.Taken together,our data suggest that porphyra-334 inhibits UV-induced apoptosis in HaCaT cells through attenuation of the caspase pathway.
